E ISSN: 2583-049X
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International Journal of Advanced Multidisciplinary Research and Studies

Volume 2, Issue 5, 2022

Applications of nanotechnology in improving plant tissue culture media



Author(s): SAM Hassan, Nagwa Zaied SM

Abstract:

Plant tissue culture focuses on the development of plant cells or plant parts, bulk propagation, genetic modification, the generation of bioactive compounds, and enhancement. Recently, using nanoparticles has been shown to be effective in removing microbial contaminants from explants, as well as demonstrating NPs' beneficial effects on induction of calluses, genetic transformation, organogenesis, somaclonal variation, somatic embryogenesis, and secondary metabolite synthesis processes that occur in the plants. The goal of this review is to summarize all the existing successes gained via nanotechnology's incorporation into plant tissue culture and to emphasize the benefits of employing nanoparticles in tissue culture. The benefits and drawbacks of employing nanoparticles in culture media are examined and given. Toxicity and safety concerns associated with nanoparticle exposure to plants and the environment have been observed. Finally, consider the future are offered using not only Ag, TiO2, and ZnO NPs, but also graphene, carbon nanotubes, SiO2, quantum dots, and dendrimers. Nanomaterials such as nano silver and TiO2 have the ability to remove microbial contamination from plant tissue culture, The effectiveness of microbial contaminates tissue culture of the plant depends on their dimensions, size, distribution and type. In addition, utilizing Ag NPs in tissue culture media increased growth parameters in vitro and increased total phenolic compounds and flavonoids accumulation in MS medium enriched with Au-Ag NPs.


Keywords: Nanomaterials, Nanoparticles, Callus induction, Shoot proliferation, Tissue culture

Pages: 382-391

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