Antioxidant and Antimicrobial Activity of Soxhlet Methanolic Seeds Extraction for Five Cassia Species

The current study was conducted in the laboratories of the Sciences College/University of Kufa and Al-Ameen Laboratory/Holy Shrine through two aspects, the methanolic extracts for five species of the Cassia genus (C. nodosa, C. surattensis, C.didymobotrya, C. fistula C. gluca) in Iraq during September 2022 - March 2023. As for the biological activity test, it included the methanol extract from the dry seeds using the soxhelt device and the preparation of the concentrations (100,200,300 and 400) mg ml-1. The well diffusion method used to test the evaluation of antibiotics activity against studied bacterial isolates (Staphylococcus aureus Klebsilla pnemonia. Psedomnus earoginosa, Escherichia.coli). Bacteria resistant (MDR) to antibiotics (Tetracyclin) and (Cephalexin) were. The results showed the biological effectiveness of the methanolic extract from the seeds of the (CASSIA) plant. It was observed that the best antibacterial activity of the type (C.fistula) and a concentration of 400 mg/ml (l) against the bacteria (s. aureus) was the most effective, as the diameter of inhibition was 27 mm, while the least diameter of inhibition of the previously mentioned species extracts was for the type (C.fistula) against the bacteria Klebsilla, where it was zero. The antioxidant activity using the DPPH method at concentrations 100 mg/ml, 0.75 mg/ml. 0.50mg/ml 0.25mg/ml. It also showed that there were significant differences between the concentration of the extract and Cassia spp. There was a strong removal of free radicals at the concentration of 100 mg/ml of C. didomoboteria plant it was 90.206% and the lowest percentage free radicals scavenge was 58.598% at 0.25 mg/ml with C.surinteesis 58.598% at the least significant difference LSD equal to 5.119.