Antibacterial Activity of Silver Nanoparticles against Acinetobacter baumannii Isolated from Different Clinical Specimens

In this study Six hundred samples were collected during the period from October 2018 to Mars 2019 from Medical city of Marjan Hospital in Province of Babylon. The clinical specimens included burn, blood, wound and urine. The samples stood principally cultured onto blood, MacConkey and Chrom agar plates used for isolation and they were incubated at 37C for 24-48 hours. Utilizing morphological characteristics, the bacterial isolates that developed as a pure and predominant growth from clinical samples were recognized. And biochemical tests in addition to Vitek2 system. In this study, a total of 20/600 (3.33%) isolates were identified as Acinetobacter baumannii, 485/ 600 (80.83%) isolates were recognized as a different bacterial species 95/600 (15.83%) of samples showed no bacterial growth. This training investigates the effectiveness of silver nanoparticles (AgNPs) against 20 A.baumannii isolates. Antibacterial activity was examined by disk diffusion assay using different concentrations of (500, 250, 125, 62.5, 31.2, 15.6 μg/ml) and each isolate's MIC and MBC values were established. With an increase in inhibition zone diameter that is directly inversely proportional to the rise in nanoparticle concentration, AgNPs demonstrated potent wide spectrum antibacterial action against the tested microorganisms. AgNPs had a MIC between 62.5 and 250 g/ml and an MBC between 125 and 500 g/ml.